CALCULATION OF ISOELECTRIC ZONES AND ISOELECTRIC POINTS
نویسندگان
چکیده
منابع مشابه
Isoelectric points of viruses.
Viruses as well as other (bio-)colloids possess a pH-dependent surface charge in polar media such as water. This electrostatic charge determines the mobility of the soft particle in an electric field and thus governs its colloidal behaviour which plays a major role in virus sorption processes. The pH value at which the net surface charge switches its sign is referred to as the isoelectric point...
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Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like beta-lactamases, appeared to be important in the case of cephalosporinases and...
متن کاملIsoelectric points of multi-domain proteins
Although the distribution of protein isoelectric points is multi-modal, large proteins show isoelectric points less variable than small proteins and their isoelectric points tend to converge to a unique value, close to the pH of the milieu in which the proteins are functional, as far as the protein dimension increases. This study demonstrates that large proteins, which contain more than a singl...
متن کاملIsoelectric Points for the Mycelium of Fungi
1. Mycelium of Rhizopus nigricans when stained with certain acid and basic dyes and washed with buffer mixtures of 0.1 M phosphoric acid and sodium hydroxide responded much like an amphoteric colloid with an isoelectric point near pH 5.0. 2. When grown on potato dextrose agar the reaction of which was varied with phosphoric acid the extent of colony growth of Rhizopus nigricans plotted against ...
متن کاملThe Isoelectric Points of Threonine and Some Related Compounds
In connection with attempts in this laboratory to increase the yields of threonine in isolations from various prtiteins, it recently became essential to determine its isoelectric point. During the time since the isolation and identification of threonine by Rose and coworkers (1) and its subsequent synthesis by West and Carter (2), the isoelectric point of the pure compound has not been reported...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1936
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)74809-6